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验证数据展示

  • FC experiment of PC-3 using CL488-82965

    FC experiment of PC-3 using CL488-82965

    1x10^6 PC-3 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Pgm5 Recombinant Antibody (CL488-82965, Clone:230206C7)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated PGM5 Recombinant antibody

PGM5 Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. CL488-82965
CloneNo. 230206C7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

PGMRP, Aciculin, 230206C7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inPC-3 cells
For other applications, we recommend the unconjugated version of this antibody, 82965-1-PBS

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82965 targets PGM5 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen PGM5 fusion protein Ag34399 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name phosphoglucomutase 5
Synonyms PGMRP, Aciculin, 230206C7
Calculated Molecular Weight 62 kDa
Observed Molecular Weight62 kDa
GenBank Accession NumberNM_021965
Gene Symbol PGM5
Gene ID (NCBI) 5239
RRIDAB_3673156
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15124
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phosphoglucomutase-like protein 5 (also known as aciculin) is an enzyme encoded by the PGM5 gene. Gene functional studies show that PGM5 is similar to PGM1 but lacks enzymatic activity. PGM5 is tightly associated with the actin cytoskeleton and has primarily been investigated as an adhesion protein. It also functions as a cytoskeletal component of cell-matrix and cell-cell contacts in muscle and non-muscle cells. (PMID: 35819729)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 PGM5 antibody CL488-82965Download protocol
Standard Protocols
Click here to view our Standard Protocols