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PGPEP1 Polyclonal antibody

PGPEP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19566-1-AP

Print datasheet

Synonyms

5 oxoprolyl peptidase, PAP I, Pcp, PGP, PGP I, PGPEP1, PGPI, Pyroglutamyl aminopeptidase I, Pyroglutamyl peptidase 1, pyroglutamyl peptidase I

验证数据展示

  • WB analysis of human brain using 19566-1-AP

    WB analysis of human brain using 19566-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 19566-1-AP (PGPEP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 19566-1-AP

    IF Staining of HepG2 using 19566-1-AP

    Immunofluorescent analysis of HepG2 cells, using PGPEP1 antibody 19566-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19566-1-AP targets PGPEP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pyroglutamyl-peptidase I
Synonyms 5 oxoprolyl peptidase, PAP I, Pcp, PGP, PGP I, PGPEP1, PGPI, Pyroglutamyl aminopeptidase I, Pyroglutamyl peptidase 1, pyroglutamyl peptidase I
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 20-23 kDa
GenBank Accession NumberNM_017712
Gene Symbol PGPEP1
Gene ID (NCBI) 54858
RRIDAB_10641046
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NXJ5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PGPEP1, also named as PGPI, belongs to the peptidase C15 family. PGPEP1 removes 5-oxoproline from various penultimate amino acid residues except L-proline.

实验方案

Product Specific Protocols
WB protocol for PGPEP1 antibody 19566-1-APDownload protocol
IF protocol for PGPEP1 antibody 19566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols