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验证数据展示

  • WB analysis of human brain using 19566-1-AP

    WB analysis of human brain using 19566-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 19566-1-AP (PGPEP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 19566-1-AP

    IF Staining of HepG2 using 19566-1-AP

    Immunofluorescent analysis of HepG2 cells, using PGPEP1 antibody 19566-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

PGPEP1 Polyclonal antibody

PGPEP1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 19566-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

5 oxoprolyl peptidase, PAP I, Pcp, PGP, PGP I, PGPEP1, PGPI, Pyroglutamyl aminopeptidase I, Pyroglutamyl peptidase 1, pyroglutamyl peptidase I

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19566-1-AP targets PGPEP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pyroglutamyl-peptidase I
别名 5 oxoprolyl peptidase, PAP I, Pcp, PGP, PGP I, PGPEP1, PGPI, Pyroglutamyl aminopeptidase I, Pyroglutamyl peptidase 1, pyroglutamyl peptidase I
计算分子量 23 kDa
观测分子量 20-23 kDa
GenBank蛋白编号NM_017712
基因名称 PGPEP1
Gene ID (NCBI) 54858
RRIDAB_10641046
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NXJ5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PGPEP1, also named as PGPI, belongs to the peptidase C15 family. PGPEP1 removes 5-oxoproline from various penultimate amino acid residues except L-proline.

实验方案

Product Specific Protocols
WB protocol for PGPEP1 antibody 19566-1-APDownload protocol
IF protocol for PGPEP1 antibody 19566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols