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  • Featured Product
  • KD/KO Validated

PGRMC1 Monoclonal antibody, PBS Only

PGRMC1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C10D11

Cat no : 66372-1-PBS

Print datasheet

Synonyms

HPR6.6, MPR, PGRMC, PGRMC1

验证数据展示

  • WB analysis of HEK-293 using 66372-1-Ig (same clone as 66372-1-PBS)

    WB analysis of HEK-293 using 66372-1-Ig (same clone as 66372-1-PBS)

    WB result of PGRMC1 antibody (66372-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PGRMC1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66372-1-PBS in a different storage buffer formulation.

  • WB analysis using 66372-1-Ig (same clone as 66372-1-PBS)

    WB analysis using 66372-1-Ig (same clone as 66372-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66372-1-Ig (PGRMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66372-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66372-1-Ig (same clone as 66372-1-PBS)

    IHC staining of human ovary tumor using 66372-1-Ig (same clone as 66372-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66372-1-Ig (PGRMC1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66372-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PGRMC1 antibody included
  • IHC staining of human ovary tumor using 66372-1-Ig (same clone as 66372-1-PBS)

    IHC staining of human ovary tumor using 66372-1-Ig (same clone as 66372-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66372-1-Ig (PGRMC1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66372-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PGRMC1 antibody included
  • IF Staining of human ovary tumor using 66372-1-Ig (same clone as 66372-1-PBS)

    IF Staining of human ovary tumor using 66372-1-Ig (same clone as 66372-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using PGRMC1 antibody (66372-1-Ig, Clone: 1C10D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66372-1-PBS in a different storage buffer formulation.

  • IF Staining of human ovary tumor using 66372-1-Ig (same clone as 66372-1-PBS)

    IF Staining of human ovary tumor using 66372-1-Ig (same clone as 66372-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using PGRMC1 antibody (66372-1-Ig, Clone: 1C10D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66372-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PGRMC1 Ready-To-Use IHC Kit. PGRMC1 primary antibody included.

产品信息

66372-1-PBS targets PGRMC1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen PGRMC1 fusion protein Ag3643 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name progesterone receptor membrane component 1
Synonyms HPR6.6, MPR, PGRMC, PGRMC1
Calculated Molecular Weight 195 aa, 22 kDa
Observed Molecular Weight 25-27 kDa
GenBank Accession NumberBC034238
Gene Symbol PGRMC1
Gene ID (NCBI) 10857
RRIDAB_2881751
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00264
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Progesterone receptor membrane component 1 (PGRMC1) is a member of a multi-protein progesterone-binding complex. However, PGRMC1 shares homology with cytochrome b5-related proteins rather than hormone receptors (PMID: 18992768). It is a heme binding protein with biding sites for Src homology (SH2) and SH3 domain-containing proteins (PMID: 17583495). PGRMC1 is overexpressed in a variety of cancers, and thus represents an important biomarker for cancer progression and a potential target for anticancer drugs (PMID: 21730960). In nonmalignant tissues, PGRMC1 is highly expressed in the liver and kidney (PMID: 9705155; 20164297).