- Featured Product
- KD/KO Validated
PGRMC1 Monoclonal antibody
PGRMC1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF-P, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1C10D11
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, fetal human brain tissue, MCF-7 cells, NIH/3T3 cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, pig brain tissue, rat brain tissue, mouse brain tissue |
Positive IHC detected in | human ovary tumor tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human ovary tumor tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IF | See 1 publications below |
产品信息
66372-1-Ig targets PGRMC1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | WB, IHC, IF-P, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human |
Immunogen | PGRMC1 fusion protein Ag3643 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | progesterone receptor membrane component 1 |
Synonyms | HPR6.6, MPR, PGRMC, PGRMC1 |
Calculated Molecular Weight | 195 aa, 22 kDa |
Observed Molecular Weight | 25-27 kDa |
GenBank Accession Number | BC034238 |
Gene Symbol | PGRMC1 |
Gene ID (NCBI) | 10857 |
RRID | AB_2881751 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | O00264 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for PGRMC1 antibody 66372-1-Ig | Download protocol |
IHC protocol for PGRMC1 antibody 66372-1-Ig | Download protocol |
IF protocol for PGRMC1 antibody 66372-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Biol Chem A progesterone receptor membrane component 1 antagonist induces large vesicles independent of progesterone receptor membrane component 1 expression. |