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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PGRMC1 Monoclonal antibody

PGRMC1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C10D11

Cat No : CL488-66372

Print datasheet

Synonyms

HPR6.6, MPR, PGRMC, PGRMC1

验证数据展示

  • FC experiment of HEK-293 using CL488-66372

    FC experiment of HEK-293 using CL488-66372

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PGRMC1 (CL488-66372, Clone:1C10D11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy PGRMC1 Ready-To-Use IHC Kit. PGRMC1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66372-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66372 targets PGRMC1 in FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen PGRMC1 fusion protein Ag3643 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name progesterone receptor membrane component 1
Synonyms HPR6.6, MPR, PGRMC, PGRMC1
Calculated Molecular Weight 195 aa, 22 kDa
Observed Molecular Weight 25-27 kDa
GenBank Accession NumberBC034238
Gene Symbol PGRMC1
Gene ID (NCBI) 10857
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00264
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Progesterone receptor membrane component 1 (PGRMC1) is a member of a multi-protein progesterone-binding complex. However, PGRMC1 shares homology with cytochrome b5-related proteins rather than hormone receptors (PMID: 18992768). It is a heme binding protein with biding sites for Src homology (SH2) and SH3 domain-containing proteins (PMID: 17583495). PGRMC1 is overexpressed in a variety of cancers, and thus represents an important biomarker for cancer progression and a potential target for anticancer drugs (PMID: 21730960). In nonmalignant tissues, PGRMC1 is highly expressed in the liver and kidney (PMID: 9705155; 20164297).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 PGRMC1 antibody CL488-66372Download protocol
Standard Protocols
Click here to view our Standard Protocols