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PGRMC2 Polyclonal antibody

PGRMC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24575-1-AP

Print datasheet

Synonyms

PMBP, DG6

验证数据展示

  • WB analysis using 24575-1-AP

    WB analysis using 24575-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24575-1-AP (PGRMC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 24575-1-AP

    WB analysis using 24575-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24575-1-AP (PGRMC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 24575-1-AP

    IHC staining of human placenta using 24575-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24575-1-AP (PGRMC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PGRMC2 antibody included
  • IHC staining of human placenta using 24575-1-AP

    IHC staining of human placenta using 24575-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24575-1-AP (PGRMC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PGRMC2 antibody included
  • IF Staining of HeLa using 24575-1-AP

    IF Staining of HeLa using 24575-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PGRMC2 antibody (24575-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, SKOV-3 cells, human placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PGRMC2 Ready-To-Use IHC Kit. PGRMC2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24575-1-AP targets PGRMC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen PGRMC2 fusion protein Ag20077 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name progesterone receptor membrane component 2
Synonyms PMBP, DG6
Calculated Molecular Weight 223 aa, 24 kDa
Observed Molecular Weight22 kDa, 28 kDa
GenBank Accession NumberBC016692
Gene Symbol PGRMC2
Gene ID (NCBI) 10424
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15173
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Membrane-associated progesterone receptor component 2 (PGRMC2), also known as DG6 or PMBP, belongs to the cytochrome b5 family. The gene of PGRMC2 maps to chromosome 4q26, and encodes a 223-amino acid single-pass membrane protein with a cytochrome b5 heme-binding domain in its cytoplasmic domain. PGRMC2 is highly homologous to PGRMC1. PGRMC2 might play a role in cancer.

实验方案

Product Specific Protocols
WB protocol for PGRMC2 antibody 24575-1-APDownload protocol
IHC protocol for PGRMC2 antibody 24575-1-APDownload protocol
IF protocol for PGRMC2 antibody 24575-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols