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PGRMC2 Polyclonal antibody

PGRMC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24575-1-AP

Print datasheet

Synonyms

PMBP, DG6

验证数据展示

  • WB analysis using 24575-1-AP

    WB analysis using 24575-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24575-1-AP (PGRMC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 24575-1-AP

    WB analysis using 24575-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24575-1-AP (PGRMC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 24575-1-AP

    IHC staining of human placenta using 24575-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24575-1-AP (PGRMC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PGRMC2 antibody included
  • IHC staining of human placenta using 24575-1-AP

    IHC staining of human placenta using 24575-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24575-1-AP (PGRMC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PGRMC2 antibody included
  • IF Staining of HeLa using 24575-1-AP

    IF Staining of HeLa using 24575-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PGRMC2 antibody (24575-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, SKOV-3 cells, human placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PGRMC2 Ready-To-Use IHC Kit. PGRMC2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24575-1-AP targets PGRMC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen PGRMC2 fusion protein Ag20077 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name progesterone receptor membrane component 2
Synonyms PMBP, DG6
Calculated Molecular Weight 223 aa, 24 kDa
Observed Molecular Weight22 kDa, 28 kDa
GenBank Accession NumberBC016692
Gene Symbol PGRMC2
Gene ID (NCBI) 10424
RRIDAB_3669443
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15173
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Membrane-associated progesterone receptor component 2 (PGRMC2), also known as DG6 or PMBP, belongs to the cytochrome b5 family. The gene of PGRMC2 maps to chromosome 4q26, and encodes a 223-amino acid single-pass membrane protein with a cytochrome b5 heme-binding domain in its cytoplasmic domain. PGRMC2 is highly homologous to PGRMC1. PGRMC2 might play a role in cancer.

实验方案

Product Specific Protocols
WB protocol for PGRMC2 antibody 24575-1-APDownload protocol
IHC protocol for PGRMC2 antibody 24575-1-APDownload protocol
IF protocol for PGRMC2 antibody 24575-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols