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验证数据展示

  • IF Staining of human cervical cancer using CL594-60249

    IF Staining of human cervical cancer using CL594-60249

    Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using CoraLite®594 PGRMC2 antibody (CL594-60249, Clone: 5F10D7 ) at dilution of 1:200.

  • IF Staining of human cervical cancer using CL594-60249

    IF Staining of human cervical cancer using CL594-60249

    Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using CoraLite®594 PGRMC2 antibody (CL594-60249, Clone: 5F10D7 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL594-60249

    FC experiment of HepG2 using CL594-60249

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PGRMC2 (CL594-60249, Clone:5F10D7) (red), or 0.4 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated PGRMC2 Monoclonal antibody

PGRMC2 Monoclonal Antibody for IF-P, FC (Intra)
Cat No. CL594-60249

产品说明书

CloneNo. 5F10D7

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

IF-P, FC (Intra)

别名

DG6, PGRMC2, PMBP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman cervical cancer tissue
Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PGRMC2 Ready-To-Use IHC Kit. PGRMC2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60249-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60249 targets PGRMC2 in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 PGRMC2 fusion protein Ag20077 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 progesterone receptor membrane component 2
别名 DG6, PGRMC2, PMBP
计算分子量 223 aa, 24 kDa
观测分子量 24+28 kDa
GenBank蛋白编号BC016692
基因名称 PGRMC2
Gene ID (NCBI) 10424
RRIDAB_2919905
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO15173
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Membrane-associated progesterone receptor component 2 (PGRMC2), also known as DG6 or PMBP, belongs to the cytochrome b5 family. This protein might play a role in cancer. The gene of PGRMC2 maps to chromosome 4q26, and encodes a 223-amino acid single-pass membrane protein with a cytochrome b5 heme-binding domain in its cytoplasmic domain. PGRMC2 has a calculated molecular weight of 24 kDa. The band of about 28 kDa detected by this monoclonal antibody is unknown but may represent phosphorylated form of PGRMC2 (PMID: 28104494).

实验方案

Product Specific Protocols
IF protocol for CL594 PGRMC2 antibody CL594-60249Download protocol
FC protocol for CL594 PGRMC2 antibody CL594-60249Download protocol
Standard Protocols
Click here to view our Standard Protocols