CoraLite®594-conjugated PHGDH Monoclonal antibody
PHGDH Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1E8B8
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PHGDH Ready-To-Use IHC Kit. PHGDH primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67591-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67591 targets PHGDH in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | PHGDH fusion protein Ag6877 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | phosphoglycerate dehydrogenase |
| Synonyms | EC:1.1.1.37, D-3-phosphoglycerate dehydrogenase, 3-PGDH, 3PGDH, 3 PGDH |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC000303 |
| Gene Symbol | PHGDH |
| Gene ID (NCBI) | 26227 |
| RRID | AB_2920161 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O43175 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PHGDH(D-3-phosphoglycerate dehydrogenase) is also named as 3-PGDH, PGDH3 and belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. It catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. 3-PGDH deficiency is a rare recessive inborn error in the biosynthesis of the amino acid L-serine characterized clinically by congenital microcephaly, psychomotor retardation, and intractable seizures(PMID:19235232 ).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 PHGDH antibody CL594-67591 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |