验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, A375 cells, Jurkat cells, MCF-7 cells |
| Positive IP detected in | A375 cells |
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20933-1-AP targets PHIP in WB, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, ChIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | PHIP fusion protein Ag14968 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pleckstrin homology domain interacting protein |
| 别名 | ndrp, PH interacting protein, PHIP |
| 计算分子量 | 1821 aa, 207 kDa |
| 观测分子量 | 207 kDa |
| GenBank蛋白编号 | BC008909 |
| 基因名称 | PHIP |
| Gene ID (NCBI) | 55023 |
| RRID | AB_10733522 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WWQ0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PHIP is a protein that binds to the insulin receptor substrate 1 protein and regulates glucose transporter translocation in skeletal muscle cells. PHIP may also regulate growth and survival of pancreatic beta cells. Elevated copy number of this gene may be associated with melanoma severity and the encoded protein may promote melanoma metastasis in human patients.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PHIP antibody 20933-1-AP | Download protocol |
| IF protocol for PHIP antibody 20933-1-AP | Download protocol |
| IP protocol for PHIP antibody 20933-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Exome Sequencing Identifies Genes and Gene Sets Contributing to Severe Childhood Obesity, Linking PHIP Variants to Repressed POMC Transcription. | ||
Cell Commun Signal RepID represses megakaryocytic differentiation by recruiting CRL4A-JARID1A at DAB2 promoter | ||
BMC Cancer LINC00955 suppresses colorectal cancer growth by acting as a molecular scaffold of TRIM25 and Sp1 to Inhibit DNMT3B-mediated methylation of the PHIP promoter |
