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验证数据展示

  • IF Staining of MCF-7 using CL488-20933

    IF Staining of MCF-7 using CL488-20933

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 PHIP antibody (CL488-20933) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of MCF-7 using CL488-20933

    FC experiment of MCF-7 using CL488-20933

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated PHIP Polyclonal antibody (CL488-20933)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated PHIP Polyclonal antibody

PHIP Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-20933

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

ndrp, PH interacting protein, PHIP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Positive FC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 20933-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-20933 targets PHIP in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen PHIP fusion protein Ag14968 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pleckstrin homology domain interacting protein
Synonyms ndrp, PH interacting protein, PHIP
Calculated Molecular Weight 1821 aa, 207 kDa
Observed Molecular Weight 207 kDa
GenBank Accession NumberBC008909
Gene Symbol PHIP
Gene ID (NCBI) 55023
RRIDAB_3672722
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WWQ0
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PHIP is a protein that binds to the insulin receptor substrate 1 protein and regulates glucose transporter translocation in skeletal muscle cells. PHIP may also regulate growth and survival of pancreatic beta cells. Elevated copy number of this gene may be associated with melanoma severity and the encoded protein may promote melanoma metastasis in human patients.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PHIP antibody CL488-20933Download protocol
FC protocol for CL Plus 488 PHIP antibody CL488-20933Download protocol
Standard Protocols
Click here to view our Standard Protocols