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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 20933-1-AP (same clone as 20933-1-PBS)

    WB analysis using 20933-1-AP (same clone as 20933-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20933-1-AP (PHIP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20933-1-PBS in a different storage buffer formulation.

  • IP experiment of A375 using 20933-1-AP (same clone as 20933-1-PBS)

    IP experiment of A375 using 20933-1-AP (same clone as 20933-1-PBS)

    IP result of anti-PHIP (IP:20933-1-AP, 4ug; Detection:20933-1-AP 1:3000) with A375 cells lysate 3600ug. This data was developed using the same antibody clone with 20933-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 20933-1-AP (same clone as 20933-1-PBS)

    IF Staining of MCF-7 using 20933-1-AP (same clone as 20933-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 20933-1-AP (PHIP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20933-1-PBS in a different storage buffer formulation.

PHIP Polyclonal antibody, PBS Only

PHIP Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 20933-1-PBS

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

20933-1-PBS targets PHIP in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen PHIP fusion protein Ag14968 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pleckstrin homology domain interacting protein
Synonyms
Calculated Molecular Weight 1821 aa, 207 kDa
Observed Molecular Weight 207 kDa
GenBank Accession NumberBC008909
Gene Symbol PHIP
Gene ID (NCBI) 55023
RRIDAB_10733522
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WWQ0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PHIP is a protein that binds to the insulin receptor substrate 1 protein and regulates glucose transporter translocation in skeletal muscle cells. PHIP may also regulate growth and survival of pancreatic beta cells. Elevated copy number of this gene may be associated with melanoma severity and the encoded protein may promote melanoma metastasis in human patients.