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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PHLPP Polyclonal antibody

PHLPP Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-22789

Print datasheet

Synonyms

hSCOP, KIAA0606, PHLPP, PHLPP1, PLEKHE1, SCOP

验证数据展示

  • IF Staining of HeLa using CL488-22789

    IF Staining of HeLa using CL488-22789

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PHLPP antibody (CL488-22789) at dilution of 1:400.

经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 22789-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-22789 targets PHLPP in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen PHLPP fusion protein Ag18794 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PH domain and leucine rich repeat protein phosphatase
Synonyms hSCOP, KIAA0606, PHLPP, PHLPP1, PLEKHE1, SCOP
Calculated Molecular Weight 1717 aa, 184 kDa
Observed Molecular Weight 185 kDa
GenBank Accession NumberBC126277
Gene Symbol PHLPP
Gene ID (NCBI) 23239
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60346
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PHLPP (PH domain leucine-rich-repeats protein phosphatase) belongs to a novel family of Ser/Thr protein phosphatases that consists of PHLPP1 and PHLPP2 isoforms. It has been shown that PHLPP negatively regulates multiple oncogenic pathways by directly dephosphorylating and inactivating key signaling molecules, including Akt, S6K, and RAF1. Observed MW of PHLPP is 185 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PHLPP antibody CL488-22789Download protocol
Standard Protocols
Click here to view our Standard Protocols