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PI3 Kinase p110 Alpha Recombinant antibody

PI3 Kinase p110 Alpha Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B4

Cat no : 82796-4-RR

Print datasheet

Synonyms

PI3K p110(alpha), PIK3CA, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, p110 alpha

验证数据展示

  • WB analysis using 82796-4-RR

    WB analysis using 82796-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82796-4-RR (PIK3CA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82796-4-RR

    Affinity and Kinetic Characterization of 82796-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82796-4-RR against Human PI3 Kinase p110 Alpha were performed. The affinity constant is 0.639 nM.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, COLO 320 cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Planning an IHC experiment? We recommend our IHCeasy PIK3CA Ready-To-Use IHC Kit. PIK3CA primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82796-4-RR targets PI3 Kinase p110 Alpha in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PI3 Kinase p110 Alpha fusion protein Ag16586 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name phosphoinositide-3-kinase, catalytic, alpha polypeptide
Synonyms PI3K p110(alpha), PIK3CA, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, p110 alpha
Calculated Molecular Weight 1068 aa, 124 kDa
Observed Molecular Weight110 kDa
GenBank Accession NumberBC113601
Gene Symbol PIK3CA
Gene ID (NCBI) 5290
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42336
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).

实验方案

Product Specific Protocols
WB protocol for PI3 Kinase p110 Alpha antibody 82796-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols