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验证数据展示

  • WB analysis of MCF-7 using 27035-1-AP (same clone as 27035-1-PBS)

    WB analysis of MCF-7 using 27035-1-AP (same clone as 27035-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27035-1-AP (PI 3 Kinase p55 gamma antibody at dilution of 1:1000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 27035-1-AP (same clone as 27035-1-PBS)

    WB analysis of HEK-293 using 27035-1-AP (same clone as 27035-1-PBS)

    WB result of PI 3 Kinase p55 gamma antibody (27035-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PI 3 Kinase p55 gamma transfected HEK-293 cells. This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

  • WB analysis of HUVEC using 27035-1-AP (same clone as 27035-1-PBS)

    WB analysis of HUVEC using 27035-1-AP (same clone as 27035-1-PBS)

    WB result of PIK3R3 antibody (27035-1-AP) on whole cell extract from HUVEC targeted by shRNAs (#1, #2) or a control shRNA (NT). (Data from Dr. Fuqiang Geng, Weill Cornell Medical College). This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 27035-1-AP (same clone as 27035-1-PBS)

    IHC staining of human kidney using 27035-1-AP (same clone as 27035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27035-1-AP (PI 3 Kinase p55 gamma antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 27035-1-AP (same clone as 27035-1-PBS)

    IHC staining of human kidney using 27035-1-AP (same clone as 27035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27035-1-AP (PI 3 Kinase p55 gamma antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 27035-1-AP (same clone as 27035-1-PBS)

    IHC staining of mouse testis using 27035-1-AP (same clone as 27035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27035-1-AP (PI3 Kinase p55 Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 27035-1-AP (same clone as 27035-1-PBS)

    IHC staining of mouse kidney using 27035-1-AP (same clone as 27035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27035-1-AP (PI3 Kinase p55 Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PI3 Kinase p55 Gamma Polyclonal antibody, PBS Only

PI3 Kinase p55 Gamma Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27035-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

27035-1-PBS targets PI3 Kinase p55 Gamma in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 PI3 Kinase p55 Gamma fusion protein Ag25884 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphoinositide-3-kinase, regulatory subunit 3 (gamma)
别名
计算分子量 85 kDa
观测分子量 55 kDa
GenBank蛋白编号BC021622
基因名称 PI3 Kinase p55 Gamma
Gene ID (NCBI) 8503
RRIDAB_2880730
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92569
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PIK3R3, also named as p55PIK, belongs to the PI3K p85 subunit family. It binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During INS stimulation, it also binds to IRS-1. It is a component of a heterodimer of p110 (catalytic) and p55 (regulatory) subunits. The antibody is specific to PIK3R3.