CoraLite®594-conjugated PI3 Kinase p110 Alpha Monoclonal antibody
PI3 Kinase p110 Alpha Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2b
Reactivity
Human
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1F6A7
验证数据展示
经过测试的应用
Positive IF detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PIK3CA Ready-To-Use IHC Kit. PIK3CA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67071-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-67071 targets PI3 Kinase p110 Alpha in IF applications and shows reactivity with Human samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | Human |
Immunogen | PI3 Kinase p110 Alpha fusion protein Ag17392 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | phosphoinositide-3-kinase, catalytic, alpha polypeptide |
Synonyms | p110 alpha, PI3 Kinase p110 Alpha, PI3 kinase subunit alpha, PI3K, PI3K alpha, PIK3CA, PtdIns 3 kinase subunit alpha |
Calculated Molecular Weight | 1068 aa, 124 kDa |
Observed Molecular Weight | 110 kDa |
GenBank Accession Number | BC113601 |
Gene Symbol | PIK3CA |
Gene ID (NCBI) | 5290 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P42336 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).
实验方案
Product Specific Protocols | |
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IF protocol for CL594 PI3 Kinase p110 Alpha antibody CL594-67071 | Download protocol |
FC protocol for CL594 PI3 Kinase p110 Alpha antibody CL594-67071 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |