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PI4KA Recombinant antibody

PI4KA Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230057G2

Cat no : 82938-1-RR

Print datasheet

Synonyms

PI4-kinase alpha, PI4K-alpha, Phosphatidylinositol 4-Kinase III alpha, Phosphatidylinositol 4-kinase alpha, EC:2.7.1.67

验证数据展示

  • WB analysis using 82938-1-RR

    WB analysis using 82938-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82938-1-RR (PI4KA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 82938-1-RR

    WB analysis using 82938-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82938-1-RR (PI4KA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 82938-1-RR

    IHC staining of human placenta using 82938-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82938-1-RR (PI4KA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 82938-1-RR

    IF Staining of MCF-7 using 82938-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PI4KA antibody (82938-1-RR, Clone: 230057G2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • FC experiment of MCF-7 using 82938-1-RR

    FC experiment of MCF-7 using 82938-1-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PI4KA Recombinant antibody (82938-1-RR, Clone:230057G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82938-1-RR

    Affinity and Kinetic Characterization of 82938-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82938-1-RR against Human PI4KA were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, MCF-7 cells, HEK-293T cells, THP-1 cells, SKOV-3 cells, rat brain tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82938-1-RR targets PI4KA in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PI4KA fusion protein Ag3062 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name phosphatidylinositol 4-kinase, catalytic, alpha
Synonyms PI4-kinase alpha, PI4K-alpha, Phosphatidylinositol 4-Kinase III alpha, Phosphatidylinositol 4-kinase alpha, EC:2.7.1.67
Calculated Molecular Weight 2044 aa, 231 kDa
Observed Molecular Weight 231 kDa
GenBank Accession NumberBC018120
Gene Symbol PI4KA
Gene ID (NCBI) 5297
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42356
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PI4KA, also named as PIK4, PIK4CA, belongs to the PI3/PI4-kinase family and Type III PI4K subfamily. It acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate. PI4KA plays a role in the formation of membrane complexes where HCV replication takes place. (PMID:19605471).

实验方案

Product Specific Protocols
WB protocol for PI4KA antibody 82938-1-RRDownload protocol
IHC protocol for PI4KA antibody 82938-1-RRDownload protocol
IF protocol for PI4KA antibody 82938-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols