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PI4KA Recombinant antibody, PBS Only

PI4KA Recombinant Antibody for WB, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230057G2

Cat No : 82938-1-PBS

Print datasheet

Synonyms

FLJ16556, PI4 kinase alpha, PI4K ALPHA, pi4K230, PI4KA, PIK4, PIK4CA, PtdIns 4 kinase alpha

验证数据展示

  • WB analysis using 82938-1-RR (same clone as 82938-1-PBS)

    WB analysis using 82938-1-RR (same clone as 82938-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82938-1-RR (PI4KA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82938-1-PBS in a different storage buffer formulation.

  • WB analysis using 82938-1-RR (same clone as 82938-1-PBS)

    WB analysis using 82938-1-RR (same clone as 82938-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82938-1-RR (PI4KA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82938-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 82938-1-RR (same clone as 82938-1-PBS)

    IF Staining of MCF-7 using 82938-1-RR (same clone as 82938-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PI4KA antibody (82938-1-RR, Clone: 230057G2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82938-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 82938-1-RR (same clone as 82938-1-PBS)

    IF Staining of MCF-7 using 82938-1-RR (same clone as 82938-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PI4KA antibody (82938-1-RR, Clone: 230057G2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82938-1-PBS in a different storage buffer formulation.

产品信息

82938-1-PBS targets PI4KA in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen PI4KA fusion protein Ag3062 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name phosphatidylinositol 4-kinase, catalytic, alpha
Synonyms FLJ16556, PI4 kinase alpha, PI4K ALPHA, pi4K230, PI4KA, PIK4, PIK4CA, PtdIns 4 kinase alpha
Calculated Molecular Weight 2044 aa, 231 kDa
Observed Molecular Weight 231 kDa
GenBank Accession NumberBC018120
Gene Symbol PI4KA
Gene ID (NCBI) 5297
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42356
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

PI4KA, also named as PIK4, PIK4CA, belongs to the PI3/PI4-kinase family and Type III PI4K subfamily. It acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate. PI4KA plays a role in the formation of membrane complexes where HCV replication takes place. (PMID:19605471).