• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PI4KA Recombinant antibody

PI4KA Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230057G2

Cat No : 82938-1-RR

Print datasheet

Synonyms

FLJ16556, PI4 kinase alpha, PI4K ALPHA, pi4K230, PI4KA, PIK4, PIK4CA, PtdIns 4 kinase alpha

验证数据展示

  • WB analysis using 82938-1-RR

    WB analysis using 82938-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82938-1-RR (PI4KA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 82938-1-RR

    WB analysis using 82938-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82938-1-RR (PI4KA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 82938-1-RR

    IF Staining of MCF-7 using 82938-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PI4KA antibody (82938-1-RR, Clone: 230057G2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of MCF-7 using 82938-1-RR

    IF Staining of MCF-7 using 82938-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PI4KA antibody (82938-1-RR, Clone: 230057G2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, MCF-7 cells, HEK-293T cells, THP-1 cells, SKOV-3 cells, rat brain tissue
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82938-1-RR targets PI4KA in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen PI4KA fusion protein Ag3062 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name phosphatidylinositol 4-kinase, catalytic, alpha
Synonyms FLJ16556, PI4 kinase alpha, PI4K ALPHA, pi4K230, PI4KA, PIK4, PIK4CA, PtdIns 4 kinase alpha
Calculated Molecular Weight 2044 aa, 231 kDa
Observed Molecular Weight 231 kDa
GenBank Accession NumberBC018120
Gene Symbol PI4KA
Gene ID (NCBI) 5297
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42356
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PI4KA, also named as PIK4, PIK4CA, belongs to the PI3/PI4-kinase family and Type III PI4K subfamily. It acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate. PI4KA plays a role in the formation of membrane complexes where HCV replication takes place. (PMID:19605471).

实验方案

Product Specific Protocols
WB protocol for PI4KA antibody 82938-1-RRDownload protocol
IF protocol for PI4KA antibody 82938-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols