验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 2 publications below |
产品信息
13486-1-AP targets PIAS3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | PIAS3 fusion protein Ag4304 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein inhibitor of activated STAT, 3 |
| 别名 | ZMIZ5, Protein inhibitor of activated STAT protein 3, EC:2.3.2.-, E3 SUMO-protein transferase PIAS3, E3 SUMO-protein ligase PIAS3 |
| 计算分子量 | 628 aa, 68 kDa |
| 观测分子量 | 67 kDa |
| GenBank蛋白编号 | BC030556 |
| 基因名称 | PIAS3 |
| Gene ID (NCBI) | 10401 |
| RRID | AB_2164224 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y6X2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PIAS3(E3 SUMO-protein ligase PIAS3) is a protein of 583 amino acid, has a molecular mass of 68 kDa and belongs to a family of proteins that includes several other members(PMID:11060035). PIAS3 is an inhibitor of activated STAT3 and binds microphthalmia-associated transcription factor, which is a key DNA-binding protein, in rat basophilic leukemia cells and mouse melanocytes(PMID:11709556). The PIAS3 antibody detects PIAS3 (68 kDa) as well as its sumoylated form (85 kDa) in some normal mouse tissues, breast and brain tumor cell lines(PMID:20371673).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PIAS3 antibody 13486-1-AP | Download protocol |
| IHC protocol for PIAS3 antibody 13486-1-AP | Download protocol |
| IF protocol for PIAS3 antibody 13486-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochim Biophys Acta Mol Cell Res AMPK induces PIAS3 mediated SUMOylation of E3 ubiquitin ligase Smurf1 impairing osteogenic differentiation and traumatic heterotopic ossification | ||
Mol Med Rep Role of SUMOylation of STAT1 in tubular epithelial‑mesenchymal transition induced by high glucose | ||
Int J Mol Sci Resveratrol Enhances Temozolomide Efficacy in Glioblastoma Cells through Downregulated MGMT and Negative Regulators-Related STAT3 Inactivation | ||
Pharmacol Res PIAS3 promotes ferroptosis by regulating TXNIP via TGF-β signaling pathway in hepatocellular carcinoma
| ||
Free Radic Biol Med PIAS3 acts as a zinc sensor under zinc deficiency and plays an important role in myocardial ischemia/reperfusion injury |
