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PIGK Recombinant antibody, PBS Only

PIGK Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241989D4

Cat no : 84568-3-PBS

Print datasheet

Synonyms

hGPI8, GPI-anchor transamidase, GPI8, GPI transamidase, EC:3.-.-.-

验证数据展示

  • WB analysis using 84568-3-RR (same clone as 84568-3-PBS)

    WB analysis using 84568-3-RR (same clone as 84568-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84568-3-RR (PIGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84568-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84568-3-RR

    Affinity and Kinetic Characterization of 84568-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84568-3-RR against Human PIGK were performed. The affinity constant is 0.501 nM.

产品信息

84568-3-PBS targets PIGK in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human
Immunogen PIGK fusion protein Ag3497 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name phosphatidylinositol glycan anchor biosynthesis, class K
Synonyms hGPI8, GPI-anchor transamidase, GPI8, GPI transamidase, EC:3.-.-.-
Calculated Molecular Weight 45 kDa
Observed Molecular Weight40-45 kDa
GenBank Accession NumberBC026186
Gene Symbol PIGK
Gene ID (NCBI) 10026
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ92643
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Phosphatidylinositol glycan, class K (PIGK), is a crucial member of the glycosyl-phosphatidylinositol transamidase (GPIT) protein complex that attaches a diverse group of macromolecules to the plasma membrane of eukaryotes. The human PIGK gene is involved in the key step of transferring GPI-anchor to the respective protein molecules in the plasma membrane (PMID: 22824918). Endogenous PIGK has 2 isoforms, 45 kDa and 36 kDa (PMID: 34193731).