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验证数据展示

  • WB analysis using 84568-3-RR

    WB analysis using 84568-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84568-3-RR (PIGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84568-3-RR

    Affinity and Kinetic Characterization of 84568-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84568-3-RR against Human PIGK were performed. The affinity constant is 0.501 nM.

PIGK Recombinant antibody

PIGK Recombinant Antibody for WB, ELISA
Cat No. 84568-3-RR

Print datasheet

CloneNo. 241989D4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

hGPI8, GPI-anchor transamidase, GPI8, GPI transamidase, EC:3.-.-.-

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, K-562 cells, HeLa cells, HT-1080 cells, U-87 MG cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84568-3-RR targets PIGK in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen PIGK fusion protein Ag3497 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name phosphatidylinositol glycan anchor biosynthesis, class K
Synonyms hGPI8, GPI-anchor transamidase, GPI8, GPI transamidase, EC:3.-.-.-
Calculated Molecular Weight 45 kDa
Observed Molecular Weight40-45 kDa
GenBank Accession NumberBC026186
Gene Symbol PIGK
Gene ID (NCBI) 10026
RRIDAB_3672072
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ92643
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phosphatidylinositol glycan, class K (PIGK), is a crucial member of the glycosyl-phosphatidylinositol transamidase (GPIT) protein complex that attaches a diverse group of macromolecules to the plasma membrane of eukaryotes. The human PIGK gene is involved in the key step of transferring GPI-anchor to the respective protein molecules in the plasma membrane (PMID: 22824918). Endogenous PIGK has 2 isoforms, 45 kDa and 36 kDa (PMID: 34193731).

实验方案

Product Specific Protocols
WB protocol for PIGK antibody 84568-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols