验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, K-562 cells, HeLa cells, HT-1080 cells, U-87 MG cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84568-3-RR targets PIGK in WB, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human |
免疫原 | PIGK fusion protein Ag3497 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | phosphatidylinositol glycan anchor biosynthesis, class K |
别名 | hGPI8, GPI-anchor transamidase, GPI8, GPI transamidase, EC:3.-.-.- |
计算分子量 | 45 kDa |
观测分子量 | 40-45 kDa |
GenBank蛋白编号 | BC026186 |
基因名称 | PIGK |
Gene ID (NCBI) | 10026 |
RRID | AB_3672072 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | Q92643 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Phosphatidylinositol glycan, class K (PIGK), is a crucial member of the glycosyl-phosphatidylinositol transamidase (GPIT) protein complex that attaches a diverse group of macromolecules to the plasma membrane of eukaryotes. The human PIGK gene is involved in the key step of transferring GPI-anchor to the respective protein molecules in the plasma membrane (PMID: 22824918). Endogenous PIGK has 2 isoforms, 45 kDa and 36 kDa (PMID: 34193731).
实验方案
Product Specific Protocols | |
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WB protocol for PIGK antibody 84568-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |