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PIN1 Monoclonal antibody, PBS Only

PIN1 Monoclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F4G3

Cat no : 68127-1-PBS

Print datasheet

Synonyms

DOD, PIN1, PPIase Pin1, Rotamase Pin1, UBL5

验证数据展示

  • WB analysis using 68127-1-Ig (same clone as 68127-1-PBS)

    WB analysis using 68127-1-Ig (same clone as 68127-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68127-1-Ig (PIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68127-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 68127-1-Ig (same clone as 68127-1-PBS)

    IP experiment of HepG2 using 68127-1-Ig (same clone as 68127-1-PBS)

    IP result of anti-PIN1 (IP:68127-1-Ig, 4ug; Detection:68127-1-Ig 1:2000) with HepG2 cells lysate 1920 ug. This data was developed using the same antibody clone with 68127-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 68127-1-Ig (same clone as 68127-1-PBS)

    IF Staining of NIH/3T3 using 68127-1-Ig (same clone as 68127-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PIN1 antibody (68127-1-Ig, Clone: 1F4G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68127-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PIN1 Ready-To-Use IHC Kit. PIN1 primary antibody included.

产品信息

68127-1-PBS targets PIN1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Applications WB, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, rabbit, pig
Immunogen PIN1 fusion protein Ag0767 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name peptidylprolyl cis/trans isomerase, NIMA-interacting 1
Synonyms DOD, PIN1, PPIase Pin1, Rotamase Pin1, UBL5
Calculated Molecular Weight 18 kDa
Observed Molecular Weight18 kDa
GenBank Accession NumberBC002899
Gene Symbol PIN1
Gene ID (NCBI) 5300
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13526
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PIN1(Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. It might regulate cellular processes distinct from the cell cycle itself, such as terminal differentiation through a modulation of differentiation-specific gene expression(PMID:20801874). It colocalizes with NEK6 in the nucleus. Pin1 inhibition simultaneously blocks multiple cancer pathways, disrupts the desmoplastic and immunosuppressive TME, and upregulates PD-L1 and ENT1, rendering pancreatic ductal adenocarcinoma (PDAC) eradicable by immunochemotherapy (PMID: 34388391).