• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68127-1-Ig

    WB analysis using 68127-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68127-1-Ig (PIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IP experiment of HepG2 using 68127-1-Ig

    IP experiment of HepG2 using 68127-1-Ig

    IP result of anti-PIN1 (IP:68127-1-Ig, 4ug; Detection:68127-1-Ig 1:2000) with HepG2 cells lysate 1920 ug.

  • IF Staining of NIH/3T3 using 68127-1-Ig

    IF Staining of NIH/3T3 using 68127-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PIN1 antibody (68127-1-Ig, Clone: 1F4G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

PIN1 Monoclonal antibody

PIN1 Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 68127-1-Ig

产品说明书

CloneNo. 1F4G3

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, rabbit, pig

应用

WB, IF/ICC, IP, ELISA

别名

DOD, PIN1, PPIase Pin1, Rotamase Pin1, UBL5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IP detected inHepG2 cells
Positive IF/ICC detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy PIN1 Ready-To-Use IHC Kit. PIN1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68127-1-Ig targets PIN1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 Human, mouse, rat, rabbit, pig
免疫原 PIN1 fusion protein Ag0767 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 peptidylprolyl cis/trans isomerase, NIMA-interacting 1
别名 DOD, PIN1, PPIase Pin1, Rotamase Pin1, UBL5
计算分子量 18 kDa
观测分子量18 kDa
GenBank蛋白编号BC002899
基因名称 PIN1
Gene ID (NCBI) 5300
RRIDAB_2923654
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ13526
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIN1(Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. It might regulate cellular processes distinct from the cell cycle itself, such as terminal differentiation through a modulation of differentiation-specific gene expression(PMID:20801874). It colocalizes with NEK6 in the nucleus. Pin1 inhibition simultaneously blocks multiple cancer pathways, disrupts the desmoplastic and immunosuppressive TME, and upregulates PD-L1 and ENT1, rendering pancreatic ductal adenocarcinoma (PDAC) eradicable by immunochemotherapy (PMID: 34388391).

实验方案

Product Specific Protocols
WB protocol for PIN1 antibody 68127-1-IgDownload protocol
IF protocol for PIN1 antibody 68127-1-IgDownload protocol
IP protocol for PIN1 antibody 68127-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols