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PIN1 Monoclonal antibody

PIN1 Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1F4G3

Cat no : 68127-1-Ig

Print datasheet

Synonyms

DOD, PIN1, PPIase Pin1, Rotamase Pin1, UBL5

验证数据展示

  • WB analysis using 68127-1-Ig

    WB analysis using 68127-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68127-1-Ig (PIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IP experiment of HepG2 using 68127-1-Ig

    IP experiment of HepG2 using 68127-1-Ig

    IP result of anti-PIN1 (IP:68127-1-Ig, 4ug; Detection:68127-1-Ig 1:2000) with HepG2 cells lysate 1920 ug.

  • IF Staining of NIH/3T3 using 68127-1-Ig

    IF Staining of NIH/3T3 using 68127-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PIN1 antibody (68127-1-Ig, Clone: 1F4G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IP detected inHepG2 cells
Positive IF/ICC detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy PIN1 Ready-To-Use IHC Kit. PIN1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68127-1-Ig targets PIN1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity Human, mouse, rat, rabbit, pig
Immunogen PIN1 fusion protein Ag0767 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name peptidylprolyl cis/trans isomerase, NIMA-interacting 1
Synonyms DOD, PIN1, PPIase Pin1, Rotamase Pin1, UBL5
Calculated Molecular Weight 18 kDa
Observed Molecular Weight18 kDa
GenBank Accession NumberBC002899
Gene Symbol PIN1
Gene ID (NCBI) 5300
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13526
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIN1(Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. It might regulate cellular processes distinct from the cell cycle itself, such as terminal differentiation through a modulation of differentiation-specific gene expression(PMID:20801874). It colocalizes with NEK6 in the nucleus. Pin1 inhibition simultaneously blocks multiple cancer pathways, disrupts the desmoplastic and immunosuppressive TME, and upregulates PD-L1 and ENT1, rendering pancreatic ductal adenocarcinoma (PDAC) eradicable by immunochemotherapy (PMID: 34388391).

实验方案

Product Specific Protocols
WB protocol for PIN1 antibody 68127-1-IgDownload protocol
IF protocol for PIN1 antibody 68127-1-IgDownload protocol
IP protocol for PIN1 antibody 68127-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols