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验证数据展示

  • IF Staining of HEK-293 using CL488-68127

    IF Staining of HEK-293 using CL488-68127

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite® Plus 488 PIN1 antibody (CL488-68127, Clone: 1F4G3 ) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated PIN1 Monoclonal antibody

PIN1 Monoclonal Antibody for IF/ICC
Cat No. CL488-68127

Print datasheet

CloneNo. 1F4G3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

IF/ICC

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy PIN1 Ready-To-Use IHC Kit. PIN1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68127-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68127 targets PIN1 in IF/ICC applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen PIN1 fusion protein Ag0767 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name peptidylprolyl cis/trans isomerase, NIMA-interacting 1
Synonyms
Calculated Molecular Weight 18 kDa
Observed Molecular Weight18 kDa
GenBank Accession NumberBC002899
Gene Symbol PIN1
Gene ID (NCBI) 5300
RRIDAB_3672973
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13526
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIN1(Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. It might regulate cellular processes distinct from the cell cycle itself, such as terminal differentiation through a modulation of differentiation-specific gene expression(PMID:20801874). It colocalizes with NEK6 in the nucleus. Pin1 inhibition simultaneously blocks multiple cancer pathways, disrupts the desmoplastic and immunosuppressive TME, and upregulates PD-L1 and ENT1, rendering pancreatic ductal adenocarcinoma (PDAC) eradicable by immunochemotherapy (PMID: 34388391).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PIN1 antibody CL488-68127Download protocol
Standard Protocols
Click here to view our Standard Protocols