CoraLite®594-conjugated PINK1 Recombinant antibody

PINK1 Recombinant Antibody for FC (Intra)
Cat No. CL594-81991-4

产品说明书

CloneNo. 240449E7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

FC (Intra)

PTEN-induced putative kinase protein 1, PTEN induced putative kinase 1, PARK6, EC:2.7.11.1, BRPK

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy PINK1 Ready-To-Use IHC Kit. PINK1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81991-4-PBS

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-81991-4 targets PINK1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 PINK1 fusion protein Ag19825 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PTEN induced putative kinase 1
别名 PTEN-induced putative kinase protein 1, PTEN induced putative kinase 1, PARK6, EC:2.7.11.1, BRPK
计算分子量 581 aa, 63 kDa
观测分子量46 kDa
GenBank蛋白编号BC028215
基因名称 PINK1
Gene ID (NCBI) 65018
RRIDAB_3673530
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BXM7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PINK1 is a mitochondrial serine/threonine-protein kinase that protects cells from stress-induced mitochondrial dysfunction. The precursor of PINK1 (65 kDa) is synthesized in the cytosol and is imported into the outer membrane of mitochondria. PINK1 is further transferred into the inner membrane. The full-length PINK1 can be proteolytically processed into 52-55 kDa and 45-46 kDa forms (PMID: 18221368; 25108683; 18031932). The half-life of the mature form of PINK1 is very short and it was proposed that the proteasome is involved in its degradation (PMID: 23472196). The gene of PINK1 maps to chromosome 1p36.12. Two alternatively spliced variants exist, the shorter isoform (30 kDa) produced by alternative splicing. Mutations in the PINK1 gene cause autosomal recessive early-onset Parkinson's disease.

实验方案

Product Specific Protocols
FC protocol for CL594 PINK1 antibody CL594-81991-4Download protocol
Standard Protocols
Click here to view our Standard Protocols
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