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验证数据展示

  • FC experiment of HeLa using CL488-15713

    FC experiment of HeLa using CL488-15713

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PIP5K1A (CL488-15713) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated PIP5K1A Polyclonal antibody

PIP5K1A Polyclonal Antibody for FC (Intra)
Cat No. CL488-15713

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

PIP5K1-alpha, PIP5K1 alpha, Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha, Phosphatidylinositol 4-phosphate 5-kinase type I alpha, EC:2.7.1.68

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 15713-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15713 targets PIP5K1A in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 PIP5K1A fusion protein Ag8340 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
别名 PIP5K1-alpha, PIP5K1 alpha, Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha, Phosphatidylinositol 4-phosphate 5-kinase type I alpha, EC:2.7.1.68
计算分子量 56 kDa, 63 kDa
观测分子量 63 kDa
GenBank蛋白编号BC007833
基因名称 PIP5K1A
Gene ID (NCBI) 8394
RRIDAB_2934412
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99755
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse roles in the cellular biology of many organisms, including signal transduction, secretion and vesicular trafficking, and regulation of cytoskeleton assembly(PMID:17688436). There are three PIP5K isoforms,α, β, and γ. The nomenclature for the α and β isoforms is switched between humans and mice(PMID:22096541). There are also several splicing variants of the γ isozyme have been identified.(PMID:20945365). PIP5K1A has some isoforms produced by alternative splicing with the molecular mass of 56-63 kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 PIP5K1A antibody CL488-15713Download protocol
Standard Protocols
Click here to view our Standard Protocols