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  • Featured Product
  • KD/KO Validated

PKC Alpha Monoclonal antibody, PBS Only

PKC Alpha Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D11F4

Cat no : 66421-1-PBS

Print datasheet

Synonyms

PRKCA, PKC-A, PKCA, PKC A, EC:2.7.11.13

验证数据展示

  • WB analysis using 66421-1-Ig (same clone as 66421-1-PBS)

    WB analysis using 66421-1-Ig (same clone as 66421-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66421-1-Ig (PKC Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66421-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66421-1-Ig (same clone as 66421-1-PBS)

    WB analysis of HEK-293 using 66421-1-Ig (same clone as 66421-1-PBS)

    WB result of PKC Alpha antibody (66421-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKC Alpha transfected HEK-293 cells. This data was developed using the same antibody clone with 66421-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66421-1-Ig (same clone as 66421-1-PBS)

    WB analysis of HeLa using 66421-1-Ig (same clone as 66421-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66421-1-Ig (PKC alpha antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66421-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66421-1-Ig (same clone as 66421-1-PBS)

    IHC staining of human lung cancer using 66421-1-Ig (same clone as 66421-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66421-1-Ig (PKC alpha antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66421-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKCA antibody included
  • IHC staining of human lung cancer using 66421-1-Ig (same clone as 66421-1-PBS)

    IHC staining of human lung cancer using 66421-1-Ig (same clone as 66421-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66421-1-Ig (PKC alpha antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66421-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKCA antibody included
  • IF Staining of HeLa using 66421-1-Ig (same clone as 66421-1-PBS)

    IF Staining of HeLa using 66421-1-Ig (same clone as 66421-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PKC Alpha antibody (66421-1-Ig, Clone: 1D11F4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66421-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66421-1-Ig (same clone as 66421-1-PBS)

    FC experiment of HeLa using 66421-1-Ig (same clone as 66421-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PKC Alpha (66421-1-Ig, Clone:1D11F4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66421-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PRKCA Ready-To-Use IHC Kit. PRKCA primary antibody included.

产品信息

66421-1-PBS targets PKC Alpha in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PKC Alpha fusion protein Ag17275 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein kinase C, alpha
Synonyms PRKCA, PKC-A, PKCA, PKC A, EC:2.7.11.13
Observed Molecular Weight 77 kDa
GenBank Accession NumberBC109274
Gene Symbol PKC Alpha
Gene ID (NCBI) 5578
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17252
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCA is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.