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  • Featured Product
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PKC Alpha Monoclonal antibody

PKC Alpha Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1D11F4

Cat no : 66421-1-Ig

Print datasheet

Synonyms

PRKCA, PKC-A, PKCA, PKC A, EC:2.7.11.13

验证数据展示

  • WB analysis using 66421-1-Ig

    WB analysis using 66421-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66421-1-Ig (PKC Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 66421-1-Ig

    WB analysis of HEK-293 using 66421-1-Ig

    WB result of PKC Alpha antibody (66421-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKC Alpha transfected HEK-293 cells.

  • WB analysis of HeLa using 66421-1-Ig

    WB analysis of HeLa using 66421-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66421-1-Ig (PKC alpha antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 66421-1-Ig

    IHC staining of human lung cancer using 66421-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66421-1-Ig (PKC alpha antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRKCA antibody included
  • IHC staining of human lung cancer using 66421-1-Ig

    IHC staining of human lung cancer using 66421-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66421-1-Ig (PKC alpha antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRKCA antibody included
  • IF Staining of HeLa using 66421-1-Ig

    IF Staining of HeLa using 66421-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PKC Alpha antibody (66421-1-Ig, Clone: 1D11F4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • FC experiment of HeLa using 66421-1-Ig

    FC experiment of HeLa using 66421-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PKC Alpha (66421-1-Ig, Clone:1D11F4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, C6 cells, RAW 264.7 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PRKCA Ready-To-Use IHC Kit. PRKCA primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66421-1-Ig targets PKC Alpha in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen PKC Alpha fusion protein Ag17275 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein kinase C, alpha
Synonyms PRKCA, PKC-A, PKCA, PKC A, EC:2.7.11.13
Observed Molecular Weight 77 kDa
GenBank Accession NumberBC109274
Gene Symbol PKC Alpha
Gene ID (NCBI) 5578
RRIDAB_2881793
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17252
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCA is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

实验方案

Product Specific Protocols
WB protocol for PKC Alpha antibody 66421-1-IgDownload protocol
IHC protocol for PKC Alpha antibody 66421-1-IgDownload protocol
IF protocol for PKC Alpha antibody 66421-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Ecotoxicol Environ Saf

Arsenic interferes with spermatogenesis involving Rictor/mTORC2-mediated blood-testis barrier disruption in mice

Authors - Xiujuan Li
View Article