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PKC Alpha Recombinant antibody, PBS Only

PKC Alpha Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240926F6

Cat no : 83840-5-PBS

Print datasheet

Synonyms

PRKCA, PKC-A, PKCA, PKC A, EC:2.7.11.13

验证数据展示

  • WB analysis using 83840-5-RR (same clone as 83840-5-PBS)

    WB analysis using 83840-5-RR (same clone as 83840-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83840-5-RR (PRKCA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83840-5-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83840-5-RR (same clone as 83840-5-PBS)

    FC experiment of HepG2 using 83840-5-RR (same clone as 83840-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PKC Alpha Recombinant antibody (83840-5-RR, Clone:240926F6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83840-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83840-5-RR

    Affinity and Kinetic Characterization of 83840-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83840-5-RR against Human PKC Alpha were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy PRKCA Ready-To-Use IHC Kit. PRKCA primary antibody included.

产品信息

The immunogen of 83840-5-PBS is PKC Alpha Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PKC Alpha fusion protein Ag1025 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein kinase C, alpha
Synonyms PRKCA, PKC-A, PKCA, PKC A, EC:2.7.11.13
Observed Molecular Weight 77 kDa
GenBank Accession NumberBC109274
Gene Symbol PKC Alpha
Gene ID (NCBI) 5578
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP17252
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCA is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.