PKC Alpha Recombinant antibody

PKC Alpha Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83840-5-RR

产品说明书

CloneNo. 240926F6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

PRKCA, PKC-A, PKCA, PKC A, EC:2.7.11.13

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells, C6 cells, human platelets, rat brain tissue
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PRKCA Ready-To-Use IHC Kit. PRKCA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83840-5-RR targets PKC Alpha in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PKC Alpha fusion protein Ag1025 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein kinase C, alpha
别名 PRKCA, PKC-A, PKCA, PKC A, EC:2.7.11.13
观测分子量 77 kDa
GenBank蛋白编号BC109274
基因名称 PKC Alpha
Gene ID (NCBI) 5578
RRIDAB_3671423
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP17252
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCA is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

实验方案

Product Specific Protocols
WB protocol for PKC Alpha antibody 83840-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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