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验证数据展示

  • WB analysis using CL647-14364

    WB analysis using CL647-14364

    Various lysates were subjected to SDS PAGE followed by western blot with CL647-14364 (PKC Gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CoraLite® Plus 647-conjugated PKC Gamma Polyclonal antibody

PKC Gamma Polyclonal Antibody for WB
Cat No. CL647-14364

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB

别名

PKC gamma, PKCC, PKCG, PRKCG, Protein kinase C gamma type, protein kinase C, gamma, SCA14

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
For other applications, we recommend the unconjugated version of this antibody, 14364-1-AP

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-14364 targets PKC Gamma in WB applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB Application Description
经测试反应性 human, mouse, rat
免疫原 PKC Gamma fusion protein Ag5681 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase C, gamma
别名 PKC gamma, PKCC, PKCG, PRKCG, Protein kinase C gamma type, protein kinase C, gamma, SCA14
计算分子量 78 kDa
观测分子量 76-80 kDa
GenBank蛋白编号BC047876
基因名称 PKC Gamma
Gene ID (NCBI) 5582
RRIDAB_2920225
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP05129
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC gamma is a neuron-specific member of the classical PKCs and is activated and translocated to subcellular regions as a result of various stimuli, including diacylglycerol synthesis, increased intracellular Ca(2+) and phorbol esters. Defects in this protein have been associated with spinocerebellar ataxia type 14 (SCA14), an autosomal dominant neurodegenerative disease.

实验方案

Product Specific Protocols
WB protocol for CL Plus 647 PKC Gamma antibody CL647-14364Download protocol
Standard Protocols
Click here to view our Standard Protocols