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CoraLite® Plus 647-conjugated PKC Gamma Polyclonal antibody

PKC Gamma Polyclonal Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-14364

Print datasheet

Synonyms

PKC gamma, PKCC, PKCG, PRKCG, Protein kinase C gamma type, protein kinase C, gamma, SCA14

验证数据展示

  • WB analysis using CL647-14364

    WB analysis using CL647-14364

    Various lysates were subjected to SDS PAGE followed by western blot with CL647-14364 (PKC Gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
For other applications, we recommend the unconjugated version of this antibody, 14364-1-AP

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-14364 targets PKC Gamma in WB applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB Application Description
Tested Reactivity human, mouse, rat
Immunogen PKC Gamma fusion protein Ag5681 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase C, gamma
Synonyms PKC gamma, PKCC, PKCG, PRKCG, Protein kinase C gamma type, protein kinase C, gamma, SCA14
Calculated Molecular Weight 78 kDa
Observed Molecular Weight 76-80 kDa
GenBank Accession NumberBC047876
Gene Symbol PKC Gamma
Gene ID (NCBI) 5582
RRIDAB_2920225
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05129
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC gamma is a neuron-specific member of the classical PKCs and is activated and translocated to subcellular regions as a result of various stimuli, including diacylglycerol synthesis, increased intracellular Ca(2+) and phorbol esters. Defects in this protein have been associated with spinocerebellar ataxia type 14 (SCA14), an autosomal dominant neurodegenerative disease.

实验方案

Product Specific Protocols
WB protocol for CL Plus 647 PKC Gamma antibody CL647-14364Download protocol
Standard Protocols
Click here to view our Standard Protocols