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验证数据展示

  • IF Staining of HeLa using CL594-66493

    IF Staining of HeLa using CL594-66493

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL594-66493 (PKC iota antibody) at dilution of 1:50.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated PKC Iota Monoclonal antibody

PKC Iota Monoclonal Antibody for IF/ICC
Cat No. CL594-66493

产品说明书

CloneNo. 3A1B6

宿主/亚型

Mouse / IgG2a

种属反应性

Human, rat, mouse

应用

IF/ICC

别名

aPKC lambda/iota, DXS1179E, PKC Iota, PKCI, PRKC lambda/iota, PRKCI, Protein kinase C iota type, protein kinase C, iota

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PRKCI Ready-To-Use IHC Kit. PRKCI primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66493-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66493 targets PKC Iota in IF/ICC applications and shows reactivity with Human, rat, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, rat, mouse
免疫原 PKC Iota fusion protein Ag4990 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 protein kinase C, iota
别名 aPKC lambda/iota, DXS1179E, PKC Iota, PKCI, PRKC lambda/iota, PRKCI, Protein kinase C iota type, protein kinase C, iota
计算分子量 68 kDa
GenBank蛋白编号BC022016
基因名称 PKC Iota
Gene ID (NCBI) 5584
RRIDAB_2883596
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP41743
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The atypical protein kinase C isoform PRKC iota (PRKCI) is a member of the protein kinase C (PKC) family of serine/threonine protein kinases. PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. PRKC iota is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. PRKC iota plays a key role in cell proliferation, differentiation, and carcinogenesis, and it has been shown to be a human oncogene.

实验方案

Product Specific Protocols
IF protocol for CL594 PKC Iota antibody CL594-66493Download protocol
FC protocol for CL594 PKC Iota antibody CL594-66493Download protocol
Standard Protocols
Click here to view our Standard Protocols