- Featured Product
- KD/KO Validated
CoraLite® Plus 488-conjugated PKM2-specific Monoclonal antibody
PKM2-specific Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
IF/ICC, FC (Intra) and More (1)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1C11C7
验证数据展示
经过测试的应用
Positive IF/ICC detected in | L02 cells, HepG2 cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
产品信息
CL488-60268 targets PKM2-specific in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Cited Applications | IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | mouse |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | PKM pyruvate kinase, muscle |
Synonyms | PKM, PKM1, PK-M1, PKM1 pyruvate kinase, muscle, PKM2 |
Calculated Molecular Weight | 58 kDa |
Observed Molecular Weight | 58 kDa |
GenBank Accession Number | NM_002654 |
Gene Symbol | PKM |
Gene ID (NCBI) | 5315 |
RRID | AB_2883131 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P14618 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 488 PKM2-specific antibody CL488-60268 | Download protocol |
FC protocol for CL Plus 488 PKM2-specific antibody CL488-60268 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Mol Med (Berl) Expression of PKM2 in wound keratinocytes is coupled to angiogenesis during skin repair in vivo and in HaCaT keratinocytes in vitro |