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验证数据展示

  • WB analysis of MCF-7 using 29489-1-AP (same clone as 29489-1-PBS)

    WB analysis of MCF-7 using 29489-1-AP (same clone as 29489-1-PBS)

    WB result of PKMYT1 antibody (29489-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKMYT1 transfected MCF-7 cells. This data was developed using the same antibody clone with 29489-1-PBS in a different storage buffer formulation.

  • WB analysis using 29489-1-AP (same clone as 29489-1-PBS)

    WB analysis using 29489-1-AP (same clone as 29489-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29489-1-AP (PKMYT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29489-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PKMYT1 Polyclonal antibody, PBS Only

PKMYT1 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 29489-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

29489-1-PBS targets PKMYT1 in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原 PKMYT1 fusion protein Ag30573 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase, membrane associated tyrosine/threonine 1
别名
计算分子量 55 kDa
观测分子量60-75 kDa
GenBank蛋白编号NM_004203
基因名称 PKMYT1
Gene ID (NCBI) 9088
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99640
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PKMYT1, also known as MYT1, belongs to the WEE1 family. PKMYT1 functions to negatively regulate the Cdc2-cyclin B complexes by phosphorylating Cdc2 on threonine 14 and tyrosine 15. PKMYT1 has also been implicated in an increasing number of cancer types, including gastric cancer, non-small-cell lung cancer, hepatocellular carcinoma, glioblastoma, neuroblastoma, and colorectal cancer, in which overexpression of PKMYT1 generally correlates with poor prognosis and disease progression. PKMYT1 also has another key role in the cell cycle - regulating Golgi membrane reassembly following mitosis - and depletion of PKMYT1 leads to cell death. PKMYT1 was strongly phosphorylated during mitosis. This antibody can recognize interphase and mitotic phase PKMYT1. (PMID: 9268380, 32127662)