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PKMYT1 Monoclonal antibody

PKMYT1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D12D3

Cat No : 67806-1-Ig

Print datasheet

Synonyms

DKFZp547K1610, FLJ20093, MYT1, Myt1 kinase, PKMYT1



经过测试的应用

Positive WB detected inLNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, PC-12 cells, HSC-T6 cells, 4T1 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67806-1-Ig targets PKMYT1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IHC, WB, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen PKMYT1 fusion protein Ag30849 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name protein kinase, membrane associated tyrosine/threonine 1
Synonyms DKFZp547K1610, FLJ20093, MYT1, Myt1 kinase, PKMYT1
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberNM_004203
Gene Symbol PKMYT1
Gene ID (NCBI) 9088
RRIDAB_2918569
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99640
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein kinase, membrane associated tyrosine/threonine (PKMYT1) is a member of the Wee family of protein kinases. PKMYT1 acts as a negative regulator of the cell cycle by inactivating the CDK1-cyclinB complex through phosphorylation of Tyr14/Tyr15 and preventing cell cycle entering into mitosis at the G2/M transition. In mammalian somatic cells, PKMYT1 affects the assembly of the Golgi apparatus and endoplasmic reticulum during telophase of mitosis by inhibiting cyclin activities. Abnormal expression of PKMYT1 is closely associated with multiple types of cancers. PKMYT1 protein was mainly localized in the cytoplasm. (PMID: 31695486, PMID: 32801781)

实验方案

Product Specific Protocols
WB protocol for PKMYT1 antibody 67806-1-IgDownload protocol
IHC protocol for PKMYT1 antibody 67806-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,WB

Cell Oncol (Dordr)

ELF3 promotes gemcitabine resistance through PKMYT1/CDK1 signaling pathway in gallbladder cancer

Authors - Linhua Yang
mouseWB

Adv Sci (Weinh)

Arf1 GTPase Regulates Golgi-Dependent G2/M Transition and Spindle Organization in Oocyte Meiosis

Authors - Kun-Huan Zhang