• Featured Product
  • KD/KO验证

PKMYT1 Monoclonal antibody

PKMYT1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67806-1-Ig

产品说明书

CloneNo. 1D12D3

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat and More (1)

应用

WB, IHC, ELISA

DKFZp547K1610, FLJ20093, MYT1, Myt1 kinase, PKMYT1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inLNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, PC-12 cells, HSC-T6 cells, 4T1 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67806-1-Ig targets PKMYT1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 Human, mouse, rat
文献引用反应性human, mouse
免疫原 PKMYT1 fusion protein Ag30849 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 protein kinase, membrane associated tyrosine/threonine 1
别名 DKFZp547K1610, FLJ20093, MYT1, Myt1 kinase, PKMYT1
计算分子量 55 kDa
观测分子量 55 kDa
GenBank蛋白编号NM_004203
基因名称 PKMYT1
Gene ID (NCBI) 9088
RRIDAB_2918569
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99640
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein kinase, membrane associated tyrosine/threonine (PKMYT1) is a member of the Wee family of protein kinases. PKMYT1 acts as a negative regulator of the cell cycle by inactivating the CDK1-cyclinB complex through phosphorylation of Tyr14/Tyr15 and preventing cell cycle entering into mitosis at the G2/M transition. In mammalian somatic cells, PKMYT1 affects the assembly of the Golgi apparatus and endoplasmic reticulum during telophase of mitosis by inhibiting cyclin activities. Abnormal expression of PKMYT1 is closely associated with multiple types of cancers. PKMYT1 protein was mainly localized in the cytoplasm. (PMID: 31695486, PMID: 32801781)

实验方案

Product Specific Protocols
WB protocol for PKMYT1 antibody 67806-1-IgDownload protocol
IHC protocol for PKMYT1 antibody 67806-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,WB

Cell Oncol (Dordr)

ELF3 promotes gemcitabine resistance through PKMYT1/CDK1 signaling pathway in gallbladder cancer

Authors - Linhua Yang
mouseWB

Adv Sci (Weinh)

Arf1 GTPase Regulates Golgi-Dependent G2/M Transition and Spindle Organization in Oocyte Meiosis

Authors - Kun-Huan Zhang
Loading...