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验证数据展示

  • WB analysis using 67806-1-Ig (same clone as 67806-1-PBS)

    WB analysis using 67806-1-Ig (same clone as 67806-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67806-1-Ig (PKMYT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67806-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67806-1-Ig (same clone as 67806-1-PBS)

    IHC staining of human colon cancer using 67806-1-Ig (same clone as 67806-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67806-1-Ig (PKMYT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67806-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 67806-1-Ig (same clone as 67806-1-PBS)

    WB analysis of MCF-7 using 67806-1-Ig (same clone as 67806-1-PBS)

    WB result of PKMYT1 antibody (67806-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKMYT1 transfected MCF-7 cells. This data was developed using the same antibody clone with 67806-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PKMYT1 Monoclonal antibody, PBS Only

PKMYT1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67806-1-PBS

产品说明书

CloneNo. 1D12D3

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

DKFZp547K1610, FLJ20093, MYT1, Myt1 kinase, PKMYT1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67806-1-PBS targets PKMYT1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 PKMYT1 fusion protein Ag30849 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 protein kinase, membrane associated tyrosine/threonine 1
别名 DKFZp547K1610, FLJ20093, MYT1, Myt1 kinase, PKMYT1
计算分子量 55 kDa
观测分子量 55 kDa
GenBank蛋白编号NM_004203
基因名称 PKMYT1
Gene ID (NCBI) 9088
RRIDAB_2918569
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99640
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein kinase, membrane associated tyrosine/threonine (PKMYT1) is a member of the Wee family of protein kinases. PKMYT1 acts as a negative regulator of the cell cycle by inactivating the CDK1-cyclinB complex through phosphorylation of Tyr14/Tyr15 and preventing cell cycle entering into mitosis at the G2/M transition. In mammalian somatic cells, PKMYT1 affects the assembly of the Golgi apparatus and endoplasmic reticulum during telophase of mitosis by inhibiting cyclin activities. Abnormal expression of PKMYT1 is closely associated with multiple types of cancers. PKMYT1 protein was mainly localized in the cytoplasm. (PMID: 31695486, PMID: 32801781)