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PLA2G4A Polyclonal antibody, PBS Only

PLA2G4A Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28924-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 28924-1-AP (same clone as 28924-1-PBS)

    WB analysis using 28924-1-AP (same clone as 28924-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 28924-1-AP (PLA2G4A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28924-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 28924-1-AP (same clone as 28924-1-PBS)

    IHC staining of human colon cancer using 28924-1-AP (same clone as 28924-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28924-1-AP (PLA2G4A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28924-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 28924-1-AP (same clone as 28924-1-PBS)

    IHC staining of human colon cancer using 28924-1-AP (same clone as 28924-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28924-1-AP (PLA2G4A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28924-1-PBS in a different storage buffer formulation.

产品信息

28924-1-PBS targets PLA2G4A in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipase A2, group IVA (cytosolic, calcium-dependent)
Synonyms
Calculated Molecular Weight 749 aa, 85 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC114340
Gene Symbol PLA2G4A
Gene ID (NCBI) 5321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP47712
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PLA2G4A, also named cPLA2 (cytosolic phospholipase A2), is a highly conserved and widely expressed enzyme that can promote lipid mediator production. It is present in the cytosol of resting cells and upon activation by a variety of agonists, undergoes Ca++-directed translocation to perinuclear membranes.PLA2G4A plays a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. It has been reported that the activity and expression levels of PLA2G4A are upregulated in several neurodegenerative diseases. It is a 749 amino acid, 85 kDa protein that migrates at 100-112 kDa in polyacrylamide gels(PMID: 17459165).