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- KD/KO Validated
PAFAH Polyclonal antibody
PAFAH Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | RAW 264.7 cells, mouse spleen tissue, mouse liver tissue, RAW264.7 |
Positive IHC detected in | human tonsillitis tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | RAW 264.7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15526-1-AP targets PAFAH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | PAFAH fusion protein Ag7227 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) |
Synonyms | PLA2G7, gVIIA-PLA2, gVIIA PLA2, Group-VIIA phospholipase A2, EC:3.1.1.47 |
Calculated Molecular Weight | 50 kDa |
Observed Molecular Weight | 43-67 kDa |
GenBank Accession Number | BC038452 |
Gene Symbol | PLA2G7 |
Gene ID (NCBI) | 7941 |
RRID | AB_2878146 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q13093 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PAFAH antibody 15526-1-AP | Download protocol |
IHC protocol for PAFAH antibody 15526-1-AP | Download protocol |
IF protocol for PAFAH antibody 15526-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Cell Biol Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation. | ||
J Immunother Cancer Inhibiting PLA2G7 reverses the immunosuppressive function of intratumoral macrophages and augments immunotherapy response in hepatocellular carcinoma | ||
J Extracell Vesicles Characterization of protein, long noncoding RNA and microRNA signatures in extracellular vesicles derived from resting and degranulated mast cells. | ||
Nat Metab Elevated dietary ω-6 polyunsaturated fatty acids induce reversible peripheral nerve dysfunction that exacerbates comorbid pain conditions.
| ||
Cell Death Discov Characterizing cellular heterogeneity in fibrotic hypersensitivity pneumonitis by single-cell transcriptional analysis. |