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CoraLite® Plus 488-conjugated PAFAH Polyclonal antibody
PAFAH Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF/ICC detected in | RAW 264.7 cells |
For other applications, we recommend the unconjugated version of this antibody, 15526-1-AP
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-15526 targets PAFAH in IF/ICC applications and shows reactivity with human, mouse samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human, mouse |
Immunogen | PAFAH fusion protein Ag7227 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) |
Synonyms | Group VIIA phospholipase A2, gVIIA PLA2, LDL PLA(2), LDL PLA2, PAF 2 acylhydrolase, PAF acetylhydrolase, PAFAH, PLA2G7 |
Calculated Molecular Weight | 50 kDa |
Observed Molecular Weight | 43-67 kDa |
GenBank Accession Number | BC038452 |
Gene Symbol | PLA2G7 |
Gene ID (NCBI) | 7941 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen Affinity Purified |
UNIPROT ID | Q13093 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PAFAH(platelet-activating factor acetylhydrolase, plasma) is also named as PLA2G7(phospholipase A2, group VII), Lp-PLA2 (Lipoprotein-associated phospholipase A2), LDL-PLA2, LDL-PLA and belongs to the AB hydrolase superfamily and lipase family. It is an enzyme which catalyzes the hydrolysis of acetyl ester at the sn-2 position of PAF. The family of PAF-Ahs consists of two intracellular isoforms (Ib and II), and one secreted isoform (plasma). Plasma PAF-AH is a monomeric polypeptide with a molecular weight of 45 kDa and it can show broad bands with a molecular weight of 43-67 kDa that it can be extensively N-glycosylated(PMID:12547653). Defects in PLA2G7 are the cause of platelet-activating factor acetylhydrolase deficiency (PAFAD), susceptibility to asthma (ASTHMA), susceptibility to atopic hypersensitivity (ATOPY).
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 PAFAH antibody CL488-15526 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |