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Species Reactivity
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验证数据展示

  • WB analysis of MCF-7 using 12529-1-AP (same clone as 12529-1-PBS)

    WB analysis of MCF-7 using 12529-1-AP (same clone as 12529-1-PBS)

    WB result of PLAA antibody (12529-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLAA transfected MCF-7 cells. This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 12529-1-AP (same clone as 12529-1-PBS)

    WB analysis of K-562 using 12529-1-AP (same clone as 12529-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 12529-1-AP (PLAA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 12529-1-AP (same clone as 12529-1-PBS)

    IHC staining of human lung cancer using 12529-1-AP (same clone as 12529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PLAA antibody included
  • IHC staining of human lung cancer using 12529-1-AP (same clone as 12529-1-PBS)

    IHC staining of human lung cancer using 12529-1-AP (same clone as 12529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PLAA antibody included
  • IP experiment of K-562 using 12529-1-AP (same clone as 12529-1-PBS)

    IP experiment of K-562 using 12529-1-AP (same clone as 12529-1-PBS)

    IP result of anti-PLAA (IP:12529-1-AP, 4ug; Detection:12529-1-AP 1:600) with K-562 cells lysate 3200ug. This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PLAA Polyclonal antibody, PBS Only

PLAA Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12529-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PLAA Ready-To-Use IHC Kit. PLAA primary antibody included.

产品信息

12529-1-PBS targets PLAA in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 PLAA fusion protein Ag3224 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase A2-activating protein
别名
计算分子量 738 aa, 81 kDa
观测分子量 72-74 kDa
GenBank蛋白编号BC032551
基因名称 PLAA
Gene ID (NCBI) 9373
RRIDAB_2164881
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y263
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Phospholipase A-2-activating protein (PLAA), also named PLAP, can interact with ubiquitin and is involved in the maintenance of ubiquitin levels. PLAA contains multiple consensus domains including WD repeats, PFU domain and PUL domain. Although the function of PLAA remains unclear, ubiquitin binding of PLAA might be the central role that connects ubiquitination and degradation in ERAD. Recent finding revealed that PLAA served as a novel nociveptive mediator after incision, and its expression level is regulated by miR-203.