验证数据展示
经过测试的应用
Positive WB detected in | Caco-2 cells, rabbit testis tissue, Caco-2. COLO320, HepG2, MOLT-4, Ramos, HL-60 |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68606-1-Ig targets PLAGL2 in WB, ELISA applications and shows reactivity with human, rabbit samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human, rabbit |
免疫原 | PLAGL2 fusion protein Ag30227 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | pleiomorphic adenoma gene-like 2 |
别名 | ZNF900, Pleiomorphic adenoma-like protein 2, KIAA0198, 3C2E5 |
计算分子量 | 496 aa, 55 kDa |
观测分子量 | 50-55 kDa |
GenBank蛋白编号 | BC023655 |
基因名称 | PLAGL2 |
Gene ID (NCBI) | 5326 |
RRID | AB_3085301 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q9UPG8 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLAGL2, also named KIAA0198, belongs to the krueppel C2H2-type zinc-finger protein family. It shows weak transcriptional activatory activity. PLAGL2, a proto-oncogene, is implicated in a variety of cancers including acute myeloid leukemia (AML), malignant glioma, colon cancer, and lung adenocarcinoma. PLAGL2 can function as a tumor suppressor by initiating cell cycle arrest and apoptosis. The calculated molecular weight of PLAGL2 is 55 kDa, but the SUMO1-modifIed form is 68 kDa (PMID: 30535429).
实验方案
Product Specific Protocols | |
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WB protocol for PLAGL2 antibody 68606-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |