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PLAGL2 Monoclonal antibody

PLAGL2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3C2E5

Cat no : 68606-1-Ig

Print datasheet

Synonyms

ZNF900, Pleiomorphic adenoma-like protein 2, KIAA0198, 3C2E5

验证数据展示

  • WB analysis using 68606-1-Ig

    WB analysis using 68606-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68606-1-Ig (PLAGL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of rabbit testis using 68606-1-Ig

    WB analysis of rabbit testis using 68606-1-Ig

    rabbit testis tissue were subjected to SDS PAGE followed by western blot with 68606-1-Ig (PLAGL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 68606-1-Ig

    FC experiment of HepG2 using 68606-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PLAGL2 Monoclonal antibody (68606-1-Ig, Clone:3C2E5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inCaco-2 cells, rabbit testis tissue, Caco-2. COLO320, HepG2, MOLT-4, Ramos, HL-60
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68606-1-Ig targets PLAGL2 in WB, ELISA applications and shows reactivity with human, rabbit samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, rabbit
Immunogen PLAGL2 fusion protein Ag30227 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name pleiomorphic adenoma gene-like 2
Synonyms ZNF900, Pleiomorphic adenoma-like protein 2, KIAA0198, 3C2E5
Calculated Molecular Weight 496 aa, 55 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC023655
Gene Symbol PLAGL2
Gene ID (NCBI) 5326
RRIDAB_3085301
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UPG8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLAGL2, also named KIAA0198, belongs to the krueppel C2H2-type zinc-finger protein family. It shows weak transcriptional activatory activity. PLAGL2, a proto-oncogene, is implicated in a variety of cancers including acute myeloid leukemia (AML), malignant glioma, colon cancer, and lung adenocarcinoma. PLAGL2 can function as a tumor suppressor by initiating cell cycle arrest and apoptosis. The calculated molecular weight of PLAGL2 is 55 kDa, but the SUMO1-modifIed form is 68 kDa (PMID: 30535429).

实验方案

Product Specific Protocols
WB protocol for PLAGL2 antibody 68606-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols