验证数据展示
经过测试的应用
| Positive WB detected in | SCaBER cells, HepG2 cells, human placenta tissue, HT-1376 cells |
| Positive IHC detected in | human placenta tissue, human appendicitis tissue, human malignant melanoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ALPP/PLAP Ready-To-Use IHC Kit. ALPP/PLAP primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 1 publications below |
产品信息
60294-1-Ig targets PLAP in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | mouse, rat |
| 免疫原 | PLAP fusion protein Ag13361 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | alkaline phosphatase, placental (Regan isozyme) |
| 别名 | PLAP-1, PALP, EC:3.1.3.1, ALPP, ALP |
| 计算分子量 | 58 kDa |
| 观测分子量 | 66 kDa |
| GenBank蛋白编号 | BC009647 |
| 基因名称 | PLAP |
| Gene ID (NCBI) | 250 |
| RRID | AB_2881409 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P05187 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLAP antibody 60294-1-Ig | Download protocol |
| IHC protocol for PLAP antibody 60294-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Mol Med Glucocorticoids decreased Cx43 expression in osteonecrosis of femoral head: The effect on proliferation and osteogenic differentiation of rat BMSCs. | ||
Front Bioeng Biotechnol Extracellular vesicles secreted by human gingival mesenchymal stem cells promote bone regeneration in rat femoral bone defects | ||
Biomed Pharmacother The multi-protective effect of IL-37-Smad3 against ox-LDL induced dysfunction of endothelial cells | ||
J Biomed Mater Res A Copper ions-photo dual-crosslinked alginate hydrogel for angiogenesis and osteogenesis | ||
