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验证数据展示

  • WB analysis of HepG2 using 60294-1-Ig (same clone as 60294-1-PBS)

    WB analysis of HepG2 using 60294-1-Ig (same clone as 60294-1-PBS)

    WB result of PLAP antibody (60294-1-Ig, 1:1000) with si-control and si-PLAP transfected HepG2 cells. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 60294-1-Ig (same clone as 60294-1-PBS)

    WB analysis of HepG2 using 60294-1-Ig (same clone as 60294-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

  • WB analysis using 60294-1-Ig (same clone as 60294-1-PBS)

    WB analysis using 60294-1-Ig (same clone as 60294-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

  • WB analysis of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

    WB analysis of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

  • IHC staining of human appendicitis using 60294-1-Ig (same clone as 60294-1-PBS)

    IHC staining of human appendicitis using 60294-1-Ig (same clone as 60294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALPP/PLAP antibody included
  • IHC staining of human appendicitis using 60294-1-Ig (same clone as 60294-1-PBS)

    IHC staining of human appendicitis using 60294-1-Ig (same clone as 60294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALPP/PLAP antibody included
  • IHC staining of human malignant melanoma using 60294-1-Ig (same clone as 60294-1-PBS)

    IHC staining of human malignant melanoma using 60294-1-Ig (same clone as 60294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:4000 (under 10x lens). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALPP/PLAP antibody included
  • IHC staining of human malignant melanoma using 60294-1-Ig (same clone as 60294-1-PBS)

    IHC staining of human malignant melanoma using 60294-1-Ig (same clone as 60294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:4000 (under 40x lens). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALPP/PLAP antibody included
  • IHC staining of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

    IHC staining of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALPP/PLAP antibody included
  • Featured Product
  • KD/KO验证

PLAP Monoclonal antibody, PBS Only

PLAP Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60294-1-PBS

产品说明书

CloneNo. 2B7C9

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

PLAP-1, PALP, EC:3.1.3.1, ALPP, ALP

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ALPP/PLAP Ready-To-Use IHC Kit. ALPP/PLAP primary antibody included.

产品信息

60294-1-PBS targets PLAP in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 PLAP fusion protein Ag13361 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 alkaline phosphatase, placental (Regan isozyme)
别名 PLAP-1, PALP, EC:3.1.3.1, ALPP, ALP
计算分子量 58 kDa
观测分子量 66 kDa
GenBank蛋白编号BC009647
基因名称 PLAP
Gene ID (NCBI) 250
RRIDAB_2881409
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP05187
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies.