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PLCD1 Polyclonal antibody

PLCD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14228-1-AP

Print datasheet

Synonyms

Phospholipase C delta 1, Phospholipase C III, phospholipase C, delta 1, PLC delta 1, PLC III, PLCD1

验证数据展示

  • WB analysis of human rectum using 14228-1-AP

    WB analysis of human rectum using 14228-1-AP

    human rectum tissue were subjected to SDS PAGE followed by western blot with 14228-1-AP (PLCD1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 14228-1-AP

    IHC staining of human breast cancer using 14228-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14228-1-AP (PLCD1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 14228-1-AP

    IHC staining of human breast cancer using 14228-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14228-1-AP (PLCD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman rectum tissue, human brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14228-1-AP targets PLCD1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen PLCD1 fusion protein Ag5468 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipase C, delta 1
Synonyms Phospholipase C delta 1, Phospholipase C III, phospholipase C, delta 1, PLC delta 1, PLC III, PLCD1
Calculated Molecular Weight 86 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC050382
Gene Symbol PLCD1
Gene ID (NCBI) 5333
RRIDAB_2163278
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51178
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PLCD1 antibody 14228-1-APDownload protocol
IHC protocol for PLCD1 antibody 14228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols