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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 18355-1-AP (same clone as 18355-1-PBS)

    WB analysis using 18355-1-AP (same clone as 18355-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18355-1-AP (PLD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18355-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 18355-1-AP (same clone as 18355-1-PBS)

    IF Staining of HeLa using 18355-1-AP (same clone as 18355-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PLD1 antibody (18355-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18355-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 18355-1-AP (same clone as 18355-1-PBS)

    IHC staining of mouse heart using 18355-1-AP (same clone as 18355-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18355-1-AP (PLD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18355-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PLD1 antibody included

PLD1 Polyclonal antibody, PBS Only

PLD1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 18355-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PLD1 Ready-To-Use IHC Kit. PLD1 primary antibody included.

产品信息

18355-1-PBS targets PLD1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 PLD1 fusion protein Ag13227 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase D1, phosphatidylcholine-specific
别名
计算分子量 1074 aa, 124 kDa
观测分子量112-124 kDa
GenBank蛋白编号BC068976
基因名称 PLD1
Gene ID (NCBI) 5337
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13393
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PLD1(Phospholipase D1) belongs to the phospholipase D family. It is implicated in the release process of a variety of hormones, most likely at a late stage of exocytosis through the production of lipids that are essential for the fusion process and seem to be also essential for fast neurotransmitter release. PLD1 is primarily localized in vesicular structures such as endosomes, lysosomes, and autophagosomes.