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验证数据展示

  • WB analysis of U-937 using 66431-1-Ig (same clone as 66431-1-PBS)

    WB analysis of U-937 using 66431-1-Ig (same clone as 66431-1-PBS)

    WB result of PLEK antibody (66431-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLEK transfected U-937 cells. This data was developed using the same antibody clone with 66431-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 66431-1-Ig (same clone as 66431-1-PBS)

    IF Staining of mouse spleen using 66431-1-Ig (same clone as 66431-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using PLEK antibody (66431-1-Ig, Clone: 1D5C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66431-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 66431-1-Ig (same clone as 66431-1-PBS)

    IF Staining of mouse spleen using 66431-1-Ig (same clone as 66431-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using PLEK antibody (66431-1-Ig, Clone: 1D5C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66431-1-PBS in a different storage buffer formulation.

  • WB analysis of HL-60 using 66431-1-Ig (same clone as 66431-1-PBS)

    WB analysis of HL-60 using 66431-1-Ig (same clone as 66431-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 66431-1-Ig (PLEK Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66431-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66431-1-Ig (same clone as 66431-1-PBS)

    IHC staining of human colon cancer using 66431-1-Ig (same clone as 66431-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66431-1-Ig (PLEK Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66431-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66431-1-Ig (same clone as 66431-1-PBS)

    IHC staining of human colon cancer using 66431-1-Ig (same clone as 66431-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66431-1-Ig (PLEK Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66431-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PLEK Monoclonal antibody, PBS Only

PLEK Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66431-1-PBS

产品说明书

CloneNo. 1D5C2

宿主/亚型

Mouse / IgG1

种属反应性

human, pig, mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

P47, Platelet 47 kDa protein, pleckstrin, PLEK

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

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产品信息

66431-1-PBS targets PLEK in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, pig, mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, pig, mouse
免疫原 PLEK fusion protein Ag3187 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 pleckstrin
别名 P47, Platelet 47 kDa protein, pleckstrin, PLEK
计算分子量 350 aa, 40 kDa
观测分子量 40-47 kDa
GenBank蛋白编号BC018549
基因名称 PLEK
Gene ID (NCBI) 5341
RRIDAB_2881802
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP08567
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PLEK (pleckstrin; also known as p47) was originally identified as the major PKC substrate in platelets and later is found to be expressed in all cells of the hemopoietic system. Through two pleckstrin homology (PH) domains at its N and C termini, PLEK may interact with various protein and/or lipid ligands and serve as an intracellular adaptor/targeting protein. Predominantly cytosolic in unstimulated cells, PLEK would undergo transient redistribution to phagosomal membrane in response to stimuli. PLEK has been found to be hyperphosphorylated in diabetic mononuclear phagocytes and promote proinflammatory cytokine secretion in diabetes. (10477609, 17579087)