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PLEK Monoclonal antibody
PLEK Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, pig, mouse
Applications
WB, IHC, IF-P, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1D5C2
验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, pig spleen tissue, THP-1 cells, U-937 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse spleen tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
| IF | See 1 publications below |
产品信息
66431-1-Ig targets PLEK in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig, mouse samples.
| Tested Applications | WB, IHC, IF-P, ELISA Application Description |
| Cited Applications | IF, IHC |
| Tested Reactivity | human, pig, mouse |
| Cited Reactivity | human |
| Immunogen | PLEK fusion protein Ag3187 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | pleckstrin |
| Synonyms | P47, Platelet 47 kDa protein, pleckstrin, PLEK |
| Calculated Molecular Weight | 350 aa, 40 kDa |
| Observed Molecular Weight | 40-47 kDa |
| GenBank Accession Number | BC018549 |
| Gene Symbol | PLEK |
| Gene ID (NCBI) | 5341 |
| RRID | AB_2881802 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P08567 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLEK (pleckstrin; also known as p47) was originally identified as the major PKC substrate in platelets and later is found to be expressed in all cells of the hemopoietic system. Through two pleckstrin homology (PH) domains at its N and C termini, PLEK may interact with various protein and/or lipid ligands and serve as an intracellular adaptor/targeting protein. Predominantly cytosolic in unstimulated cells, PLEK would undergo transient redistribution to phagosomal membrane in response to stimuli. PLEK has been found to be hyperphosphorylated in diabetic mononuclear phagocytes and promote proinflammatory cytokine secretion in diabetes. (10477609, 17579087)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLEK antibody 66431-1-Ig | Download protocol |
| IHC protocol for PLEK antibody 66431-1-Ig | Download protocol |
| IF protocol for PLEK antibody 66431-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
