验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, pig spleen tissue, THP-1 cells, U-937 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse spleen tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
| IF | See 1 publications below |
产品信息
66431-1-Ig targets PLEK in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig, mouse samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | IF, IHC |
| 经测试反应性 | human, pig, mouse |
| 文献引用反应性 | human |
| 免疫原 | PLEK fusion protein Ag3187 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | pleckstrin |
| 别名 | P47, Platelet 47 kDa protein, pleckstrin, PLEK |
| 计算分子量 | 350 aa, 40 kDa |
| 观测分子量 | 40-47 kDa |
| GenBank蛋白编号 | BC018549 |
| 基因名称 | PLEK |
| Gene ID (NCBI) | 5341 |
| RRID | AB_2881802 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P08567 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLEK (pleckstrin; also known as p47) was originally identified as the major PKC substrate in platelets and later is found to be expressed in all cells of the hemopoietic system. Through two pleckstrin homology (PH) domains at its N and C termini, PLEK may interact with various protein and/or lipid ligands and serve as an intracellular adaptor/targeting protein. Predominantly cytosolic in unstimulated cells, PLEK would undergo transient redistribution to phagosomal membrane in response to stimuli. PLEK has been found to be hyperphosphorylated in diabetic mononuclear phagocytes and promote proinflammatory cytokine secretion in diabetes. (10477609, 17579087)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLEK antibody 66431-1-Ig | Download protocol |
| IHC protocol for PLEK antibody 66431-1-Ig | Download protocol |
| IF protocol for PLEK antibody 66431-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
