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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PLEK Monoclonal antibody

PLEK Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D5C2

Cat no : CL488-66431

Print datasheet

Synonyms

FLJ27168, P47, Platelet 47 kDa protein, pleckstrin, PLEK

验证数据展示

  • WB analysis using CL488-66431

    WB analysis using CL488-66431

    HL-60 cells were subjected to SDS PAGE followed by western blot with CL488-66431 (PLEK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHL-60 cells, THP-1 cells, U-937 cells
For other applications, we recommend the unconjugated version of this antibody, 66431-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66431 targets PLEK in WB applications and shows reactivity with human, pig samples.

Tested Applications WB Application Description
Tested Reactivity human, pig
Immunogen PLEK fusion protein Ag3187 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name pleckstrin
Synonyms FLJ27168, P47, Platelet 47 kDa protein, pleckstrin, PLEK
Calculated Molecular Weight 350 aa, 40 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC018549
Gene Symbol PLEK
Gene ID (NCBI) 5341
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08567
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLEK (pleckstrin; also known as p47) was originally identified as the major PKC substrate in platelets and later is found to be expressed in all cells of the hemopoietic system. Through two pleckstrin homology (PH) domains at its N and C termini, PLEK may interact with various protein and/or lipid ligands and serve as an intracellular adaptor/targeting protein. Predominantly cytosolic in unstimulated cells, PLEK would undergo transient redistribution to phagosomal membrane in response to stimuli. PLEK has been found to be hyperphosphorylated in diabetic mononuclear phagocytes and promote proinflammatory cytokine secretion in diabetes. (10477609, 17579087)

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 PLEK antibody CL488-66431Download protocol
Standard Protocols
Click here to view our Standard Protocols