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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PLEK2 Polyclonal antibody

PLEK2 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11685

Print datasheet

Synonyms

Pleckstrin-2, Pleckstrin 2

验证数据展示

  • IF Staining of HT-29 using CL488-11685

    IF Staining of HT-29 using CL488-11685

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using CoraLite® Plus 488 PLEK2 antibody (CL488-11685) at dilution of 1:200.

  • FC experiment of HT-29 using CL488-11685

    FC experiment of HT-29 using CL488-11685

    1X10^6 HT-29 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PLEK2 (CL488-11685) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHT-29 cells
Positive FC (Intra) detected inHT-29 cells
Planning an IHC experiment? We recommend our IHCeasy PLEK2 Ready-To-Use IHC Kit. PLEK2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11685-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11685 targets PLEK2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen PLEK2 fusion protein Ag2297 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pleckstrin 2
Synonyms Pleckstrin-2, Pleckstrin 2
Calculated Molecular Weight 353 aa, 40 kDa
Observed Molecular Weight 38-40 kDa
GenBank Accession NumberBC001226
Gene Symbol PLEK2
Gene ID (NCBI) 26499
RRIDAB_2919036
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NYT0
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PLEK2 antibody CL488-11685Download protocol
FC protocol for CL Plus 488 PLEK2 antibody CL488-11685Download protocol
Standard Protocols
Click here to view our Standard Protocols