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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated PLK1 Polyclonal antibody

PLK1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-10305

Print datasheet

Synonyms

PLK, PLK 1, PLK1, Polo like kinase 1, STPK13

验证数据展示

  • FC experiment of HeLa using CL647-10305

    FC experiment of HeLa using CL647-10305

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human PLK1 (CL647-10305) (red), or 0.2 ug CL647-30000 Rabbit IgG (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

PLK1 expression is low or nonexistent in normal tissues, but high in tumor tissues.

Planning an IHC experiment? We recommend our IHCeasy PLK1 Ready-To-Use IHC Kit. PLK1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10305-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-10305 targets PLK1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen PLK1 fusion protein Ag0284 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polo-like kinase 1 (Drosophila)
Synonyms PLK, PLK 1, PLK1, Polo like kinase 1, STPK13
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC002369
Gene Symbol PLK1
Gene ID (NCBI) 5347
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP53350
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLK1(Polo-like kinase 1), also named as PLK and STPK13, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and CDC5/Polo subfamily. PLK1 is a Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.The localization of Polo changes during the cell cycle. In interphase, Polo shows a mostly diffuse cytoplasmic localization. Polo becomes concentrated on centrosomes from early prophase and appears on centromeres or kinetochores from late prophase, before nuclear envelope breakdown (NEB). After anaphase onset, Polo relocalizes to the central spindle and remains enriched at the midbody ring in late stages of cytokinesis (PMID: 29167465).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 PLK1 antibody CL647-10305Download protocol
Standard Protocols
Click here to view our Standard Protocols